Device
Part:BBa_K317004:Design
Designed by: Yoshiyuki MURAKAMI Group: iGEM10_Tokyo-NoKoGen (2010-10-19)
Phototaxis Device (Atractant)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 1850 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 134
Illegal NgoMIV site found at 392
Illegal AgeI site found at 1652 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1088
Illegal BsaI.rc site found at 1367
Illegal SapI site found at 862
Illegal SapI.rc site found at 1868
Design Notes
The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication
Source
Natronomonas pharaonis (Obtained from National Institute of Technology and Evaluation(NBRC). (NBRC 14720T)) Salmonella Typhimurium
References
Jung,et al.(2001)J.Bacteriol.183,6365–6371